Rac2 Protein, Human, Recombinant (His)

カタログ番号 TMPY-02439

別名: p21-Rac2, HSPC022, Gx, EN-7, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)

Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on / off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells.

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Rac2 Protein, Human, Recombinant (His)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 161,000

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Product Properties

参考文献

説明

Species	Human
Expression Host	E. coli
Tag	His
Accession Number	P15153
別名	p21-Rac2, HSPC022, Gx, EN-7, ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2)
Construction	The Human RAC2 (P15153) (Met 1-Cys 189) was expressed, with a polyhistidine tag at the N-terminus.
Protein Purity	> 94 % as determined by SDS-PAGE
分子量	22.6 kDa (predicted)

Endotoxin	Please contact us for more information.
Formulation	Supplied as sterile PBS, 20% glycerol, pH 7.5.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store the product under sterile conditions at -20℃ to -80℃. Samples are stable for up to 12 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Ras-related C3 botulinum toxin substrate 2 (Rac2) is a small G-protein belonging to the Ras subfamily of the GTPase family. Rac2 acts as an "on / off" switch for signal transduction cascades and motilities. When GDP is attached to the small G-protein, the enzyme is inactivated. Release of the GDP and replace of the GTP cativate the GTPasee. Rac2 remains active until the GTP is hydrolyzed to GDP. Rac2 is a hematopoietic-specific Rho family GTPase implicated as an important constituent of the NADPH oxidase complex and shares 92% amino acid identity with the ubiquitously expressed Rac1. The small G-protein Rac2 regulates the rearrangements of actin and membrane necessary for Fcy receptor-mediated phagocytosis by macrophages. Activated Rac2 binds to the p21-binding domain of PAK1 and this binding provided a basis for microscopic methods to localize activation of these G proteins inside cells.

参考文献

1. Adam D, et al. (2003) Cdc42, Rac1, and Rac2 Display Distinct Patterns of Activation during Phagocytosis.Mol Biol Cell. 15 (8 ): 3509-19. 2. Walmsley MJ, et al. (2003) Critical Roles for Rac1 and Rac2 GTPases in B Cell Development and Signaling. Science. 302 (5644): 459-62. 3. Holland M, et al. (2011) RAC2, AEP, and ICAM1 expression are associated with CNS disease in a mouse model of pre-B childhood acute lymphoblastic leukemia. Blood. 118 (3): 638-49.

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Keywords

Rac2 Protein, Human, Recombinant (His) p21-Rac2 EN 7 HSPC022 HSPC-022 Gx rho family, small GTP binding protein Rac2 ras-related C3 botulinum toxin substrate 2 EN-7 EN7 ras-related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2) HSPC 022 recombinant recombinant-proteins proteins protein