R-Spondin 3/RSPO3 Protein, Human, Recombinant (aa 1-146, His)

カタログ番号 TMPY-02115

別名: CRISTIN1, THSD2, R-spondin 3, PWTSR

R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.

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R-Spondin 3/RSPO3 Protein, Human, Recombinant (aa 1-146, His)

パッケージサイズ	在庫状況	単価(税別)
50 μg	在庫あり	￥ 68,000
100 μg	約5 days	￥ 105,500
200 μg	約5 days	￥ 163,500

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生物学的特性に関する説明

Technical Params

Product Properties

参考文献

生物学的情報

Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His at 2 μg/ml (100 μl/well) can bind Human RNF43 hFc , the EC50 of Human RNF43 hFc is 3.0-12.0 ng/mL.

説明

Species	Human
Expression Host	HEK293
Tag	His
Accession Number	Q9BXY4-1
別名	CRISTIN1, THSD2, R-spondin 3, PWTSR
Construction	A DNA sequence encoding the amino acids (Met 1-Val 146) of human RSPO3 (Q9BXY4-1) was fused with a polyhistidine tag at the C-terminus.
Protein Purity	> 96 % as determined by SDS-PAGE
分子量	15.3 kDa (predicted)

Endotoxin	< 1.0 EU per μg of the protein as determined by the LAL method.
Formulation	Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0. 01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Reconstitution	A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
Stability & Storage	Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.

参考文献

1. Aoki M, et al. (2007) R-spondin3 is required for mouse placental development. Dev Biol. 301(1): 218-26. 2. Kazanskaya O, et al. (2008) The Wnt signaling regulator R-spondin 3 promotes angioblast and vascular development. Development. 135(22): 3655-64. 3. Kim KA, et al. (2008) R-Spondin family members regulate the Wnt pathway by a common mechanism. Mol Biol Cell. 19(6): 2588-96. 4. Ohkawara B, et al. (2011) Rspo3 Binds Syndecan 4 and Induces Wnt/PCP Signaling via Clathrin-Mediated Endocytosis to Promote Morphogenesis. Dev Cell. 20(3): 303-14.

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Keywords

R-Spondin 3/RSPO3 Protein, Human, Recombinant (aa 1-146, His) CRISTIN1 THSD 2 THSD2 R-spondin 3 PWTSR THSD-2 CRISTIN-1 CRISTIN 1 recombinant recombinant-proteins proteins protein