| 説明 |
DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. |
| Species |
Human
|
| Expression Host |
HEK293 Cells
|
| Tag |
C-hFc
|
| Accession Number |
O14763-1
|
| 別名 |
TRAIL R2, TNFRSF10B, TRICK2, KILLER, DR5, TRAIL receptor 2, Fas-like protein, TRICKB, ZTNFR9, DR5TRICK2B, CD262, TRICK2A, KILLER/DR5, TRAILR2
|
| Construction |
Ile56-Glu182
|
| Protein Purity |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
|
| 分子量 |
41.1 kDa (predicted). Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
|
| Endotoxin |
< 1 EU/μg by the LAL method.
|
| Formulation |
Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Tris, 100 mM NaCl (pH 7.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
|
| Reconstitution |
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
|
| Stability & Storage |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
|
| Shipping |
In general, Lyophilized powders are shipping with blue ice.
|
| Research Background |
DR5, also called TRAIL R2, TRICK 2, TNFRSF10B, and MK is a type 1 TNF R superfamily, membrane protein which is a receptor for TRAIL (APO2 ligand). DR5 is a receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis.
|
参考文献
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