keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 .
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
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1 mg | 在庫あり | ¥ 10,000 | |||
5 mg | 在庫あり | ¥ 29,000 | |||
10 mg | 在庫あり | ¥ 45,500 | |||
25 mg | 在庫あり | ¥ 80,000 | |||
50 mg | 在庫あり | ¥ 118,500 | |||
100 mg | 在庫あり | ¥ 169,000 |
説明 | Ac-DEVD-AMC (AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN) is the substrate of Caspase-3 . |
In vitro | The AMC moiety is highest at 2 h with an area?of 5.6±1.8, the AMC peak area for Ac-DEVD-AMC incubated without a lung specimen (substrate alone) for 6 h is relatively small [1]. Ac-DEVD-AMC can be used to monitor intracellular caspase-3 activity[2]. |
細胞研究 | Lung fragments (about 20 mg each) are collected from rats and mice. The samples are incubated at 37°C in 50 mL KH buffer or MEM (continuously gass with 95% O2: 5% CO2) for up to 6 h. At specific time points, samples are incubated in oxygenated KH buffer or MEM with 32 μM zVAD-fmk or 15 μL DMSO for 20 min (f/v=1.0 mL). Ac-DEVD-AMC (37 μM) is then added and the incubation continues for an additional 20 min. At the end of incubation, the tissue is disrupted by vigorous homogenization for 2 min, sonication for 3 min, and 10 passages through a 27-G needle. This disruption procedure quenches the Ac-DEVD-AMC cleavage reaction due to dilution. The supernatants are collected by centrifugation (~6,300g for 90 min) through a microcentrifuge filter, separated on HPLC, and analyzed for the fluorogenic AMC moiety [2]. |
別名 | AC-ASP-MET-GLN-ASP-AMC, AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN |
分子量 | 675.64 |
分子式 | C30H37N5O13 |
CAS No. | 169332-61-0 |
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: ≤ 10 mg/mL (14.80 mM)
H2O: < 0.1 mg/mL (insoluble)
You can also refer to dose conversion for different animals. 詳細
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Ac-DEVD-AMC 169332-61-0 Apoptosis Proteases/Proteasome Caspase Ac DEVD AMC AcDEVDAMC Inhibitor inhibit AC-ASP-MET-GLN-ASP-AMC AC-ASP-MET-GLN-ASP-7-AMINO-4-METHYLCOUMARIN inhibitor