Powder: -20°C for 3 years | In solvent: -80°C for 1 year
N6022(IC50 of 8 nM) is an effective, reversible, and selective S-Nitrosoglutathione reductase(GSNOR) inhibitor.
説明 | N6022(IC50 of 8 nM) is an effective, reversible, and selective S-Nitrosoglutathione reductase(GSNOR) inhibitor. |
ターゲット&IC50 | GSNOR:8 nM |
In vitro | N6022 binds to rat plasma proteins in a concentration-dependent manner. At lower drug concentrations (20 μM), N6022 show more effect on ATP than on GSH. N6022 (IC50 of 8 nM and a Ki of 2.5 nM) binds in the GSNO substrate binding pocket like a competitive inhibitor. N6022 is uncompetitive with cofactors NAD+ and NADH. |
In vivo | N6022 treats rats in 50 mg/kg leading to a slight increase in the incidence of granulomas. In serum, N6022 remained in solution is up to 5 mg/mL. |
細胞研究 | N6022 is dissolved in 5% DMSO. N6022 is tested using a rat hepatoma (H4IIE) cell line whereby cells are seeded into 96-well plates and cultured in medium containing 20% bovine serum. Following an equilibration period of 48 h, the cells are treated with N6022 (5% DMSO vehicle) at concentrations of 0, 1, 5, 10, 20, 50, 100, and 300 μM for 24 h at 37°C in 5% CO2. Camptothecin and rotenone are included as positive controls. The cell supernatant or the cells themselves are harvested for biochemical analysis. |
分子量 | 414.46 |
分子式 | C24H22N4O3 |
CAS No. | 1208315-24-5 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 46 mg/mL
You can also refer to dose conversion for different animals. 詳細
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N6022 1208315-24-5 Metabolism GSNOR Inhibitor N-6022 N 6022 inhibit S-nitrosoglutathione reductase inhibitor