Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Purvalanol A (NG-60) is an effective and cell-permeable CDK inhibitor with IC50 of 70/4/35/850 nM for cdk2-cyclin A/B/E, and cdk4-cyclin D1, respectively.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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サンプルについてお問い合わせ | |||||
5 mg | 在庫あり | ¥ 7,500 | |||
10 mg | 在庫あり | ¥ 10,500 | |||
25 mg | 在庫あり | ¥ 18,000 | |||
50 mg | 在庫あり | ¥ 29,000 | |||
100 mg | 在庫あり | ¥ 47,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 11,000 |
説明 | Purvalanol A (NG-60) is an effective and cell-permeable CDK inhibitor with IC50 of 70/4/35/850 nM for cdk2-cyclin A/B/E, and cdk4-cyclin D1, respectively. |
ターゲット&IC50 | CDK4-CyclinD1:850 nM, CDK2-CyclinE:35 nM, CDC2-cyclinB:4 nM, CDK2-CyclinA:70 nM |
In vivo | In MCF-7 cells, Purvalanol A leads to a 50% reduction in cell viability, while MDA-MB-231 cells exhibit lower sensitivity to Purvalanol A, showing a 32% decrease in cell viability. Purvalanol A induces mitochondrial-mediated apoptosis in both MCF-7 and MDA-MB-231 cells. The compound's effect on reducing the viability of these cell lines is dose-dependent. By inhibiting cell cycle progression and the c-Src signaling pathway, Purvalanol A effectively prevents c-Src-mediated transformation and significantly inhibits anchorage-independent growth in certain human cancer cells with upregulated c-Src. Additionally, Purvalanol A markedly suppresses anchorage-dependent growth in HT29 and SW480 human colorectal cancer cells. |
細胞研究 | Cells are seeded at 10000 density in 96-well plates and treated with various concentrations of Purvalanol A (0-100 μM) for 24 h. Cells are exposed to 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet- razolium bromide dye (5 mg/mL) and are incubated at 37℃ for 4 h. In order to solubilize the formazan crystals 100 μL DMSO is added. Absorbance is determined at 570 nm spectrophotometrically.(Only for Reference) |
別名 | NG-60 |
分子量 | 388.89 |
分子式 | C19H25ClN6O |
CAS No. | 212844-53-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 38.9 mg/mL (100 mM)
Ethanol: 19.5 mg/mL (50 mM)
You can also refer to dose conversion for different animals. 詳細
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Purvalanol A 212844-53-6 Apoptosis Autophagy Cell Cycle/Checkpoint CDK inhibit Inhibitor Cyclin dependent kinase NG-60 NG 60 NG60 inhibitor