Noggin/NOG Protein, Mouse, Recombinant (His & Flag)

Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt. Noggin/NOG Protein, Mouse, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-His-Flag tag. The predicted molecular weight is 25.16 kDa and the accession number is P97466.

Noggin/NOG Protein, Mouse, Recombinant (His & Flag)

パッケージサイズ	在庫状況	単価(税別)
100 μg	約5 days	￥ 96,000
500 μg	約5 days	￥ 383,500
1 mg	約5 days	￥ 643,000

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説明

Species	Mouse
Expression Host	HEK293
Tag	N-His-Flag
Accession Number	P97466
別名	SYNS1, NOG, SYM1, Noggin
Construction	Recombinant Mouse Noggin Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus. It contains Gln28-Cys232.[Accession \|P97466]
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
分子量	The protein has a predicted MW of 25.16 kDa. Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.

Endotoxin	Less than 1EU per μg by the LAL method.
Formulation	Lyophilized from 0.22μm filtered solution in PBS, 100mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability & Storage	-20 to -80°C for 12 months as supplied from date of receipt. -20 to -80°C for 3-6 months in unopened state after reconstitution. 2-8°C for 2-7 days after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Shipping	In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Research Background	Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.

参考文献

1. Sun Z, et al. Noggin is associated with a poor prognosis of gastric cancer by promoting the proliferation of gastric cancer cells via the upregulation of EGFR. Int J Oncol. 2020 Sep;57(3):813-824. doi: 10.3892/ijo.2020.508Epub 2020 Jun 16. PMID: 32705152.

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Noggin/NOG Protein, Mouse, Recombinant (His & Flag)

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