Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CCT007093 is an effective PPM1D (WIP1) inhibitor (IC50: 8.4 μM).
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
サンプルについてお問い合わせ | |||||
5 mg | 在庫あり | ¥ 9,000 | |||
10 mg | 在庫あり | ¥ 15,500 | |||
25 mg | 在庫あり | ¥ 29,000 | |||
50 mg | 在庫あり | ¥ 52,000 | |||
100 mg | 在庫あり | ¥ 91,500 | |||
500 mg | お問い合わせ | ¥ 248,000 |
説明 | CCT007093 is an effective PPM1D (WIP1) inhibitor (IC50: 8.4 μM). |
ターゲット&IC50 | PPM1D:8.4 μM |
In vivo | CCT007093, when used in combination with Paclitaxel, synergistically inhibits four Paclitaxel-resistant triple-negative breast cancer cell lines. CCT007093 selectively exhibits significant inhibition on human tumor cell lines over-expressing PPM1D (MCF-7, KPL-1, and MCF-3B). By activating p38 kinase activity, CCT007093 induces cell death. It selectively promotes apoptosis in breast cancer cells and ectopically expressed Wip1 in keratinocytes, while reducing UV-mediated apoptotic responses in Wip1-deficient naked cells and a skin keratinocyte model. |
キナーゼ試験 | In vitro phosphatase assay: Recombinant PPM1D (20-50?pmol) is diluted in Tris buffer (50?mM, pH 8), NaCl (100?mM), β-mercaptoethanol (1?mM) or DTT (1?mM) and treated with MnCl2 (0, 1, 10 and 20?mM) or MgCl2 (0 and 40?mM). Where appropriate, inhibitors of PPM1D (10-50?μM) are added and the assay mix incubated for 30?min at room temperature. Recombinant phospho-P38 (200?pmol) is then added and the mixture incubated at 37°C for 1?h. The reaction is quenched by the addition of excess ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulphate-sample loading buffer and boiling for 5?min at 95°C followed by gel electrophoresis and western blotting. |
細胞研究 | Cells are transfected with a pSUPER plasmid and an additional plasmid expressing the blasticidin resistance gene (pEFBsd) in a molar ratio of 10:1. Cells are plated in 10?cm plates 24?h after transfection. Blasticidin selection (5?μg/ml) is initiated 48?h post-transfection and replenished every 3 days. Colonies are fixed in methanol and stained with crystal violet after 14 days. Colonies are quantified on a Colcount and the surviving fraction (SF) determined.(Only for Reference) |
分子量 | 272.39 |
分子式 | C15H12OS2 |
CAS No. | 176957-55-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: Slightly soluble
You can also refer to dose conversion for different animals. 詳細
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CCT007093 176957-55-4 Apoptosis Autophagy Metabolism Phosphatase inhibit CCT 007093 CCT-007093 Inhibitor inhibitor