Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ergolide, a sesquiterpene lactone extracted from the dried flowers of Inula Britannica, effectively hinders the expression of inducible nitric oxide synthase and cyclo-oxygenase-2 in RAW 264.7 macrophages by deactivating NF-κB.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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1 mg | 在庫あり | ¥ 27,000 | |||
5 mg | 在庫あり | ¥ 57,500 | |||
10 mg | 在庫あり | ¥ 97,500 | |||
25 mg | 在庫あり | ¥ 159,000 | |||
50 mg | 在庫あり | ¥ 221,000 | |||
100 mg | 在庫あり | ¥ 296,000 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 68,500 |
説明 | Ergolide, a sesquiterpene lactone extracted from the dried flowers of Inula Britannica, effectively hinders the expression of inducible nitric oxide synthase and cyclo-oxygenase-2 in RAW 264.7 macrophages by deactivating NF-κB. |
In vitro | Ergolide markedly decreased the production of prostaglandin E(2) (PGE(2)) in cell-free extract of LPS/IFN-gamma-stimulated RAW 264.7 macrophages in a concentration-dependent manner, without alteration of the catalytic activity of COX-2 itself.?Ergolide decreased the level of iNOS and COX-2 protein, and iNOS mRNA caused by stimulation of LPS/IFN-gamma in a concentration-dependent manner, as measured by Western blot and Northern blot analysis, respectively.?Ergolide inhibited nuclear factor-kappaB (NF-kappaB) activation, a transcription factor necessary for iNOS and COX-2 expression in response to LPS/IFN-gamma.?This effect was accompanied by the parallel reduction of nuclear translocation of subunit p65 of NF-kappaB as well as IkappaB-alpha degradation.?In addition, these effects were completely blocked by treatment of cysteine, indicating that this inhibitory effect of ergolide could be mediated by alkylation of NF-kappaB itself or an upstream molecule of NF-kappaB.?Ergolide also directly inhibited the DNA-binding activity of active NF-kappaB in LPS/IFN-gamma-pretreated RAW 264.7 macrophages.?These results demonstrate that the suppression of NF-kappaB activation by ergolide might be attributed to the inhibition of nuclear translocation of NF-kappaB resulted from blockade of the degradation of IkappaB and the direct modification of active NF-kappaB, leading to the suppression of the expression of iNOS and COX-2,?which play important roles in inflammatory signalling pathway. |
分子量 | 306.35 |
分子式 | C17H22O5 |
CAS No. | 54999-07-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (163.21 mM), Sonication is recommended.
You can also refer to dose conversion for different animals. 詳細
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Ergolide 54999-07-4 NF-Κb NF-κB Nuclear factor-kappaB Nuclear factor-κB Inhibitor inhibit inhibitor