Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MPI-0479605 is an ATP competitive and selective inhibitor.
説明 | MPI-0479605 is an ATP competitive and selective inhibitor. |
ターゲット&IC50 | Mps1:1.8 nM |
キナーゼ試験 | In Vitro Kinase Assays and Selectivity Screening : To measure Mps1 activity, 25 ng of recombinant, full-length enzyme is incubated in reaction buffer (50 mM Tris-HCl pH 7.5, 10 mM MgCl2, 0.01% Triton X-100 and 5 μM Myelin basic protein (MBP)) containing vehicle (DMSO alone) or inhibitors. Forty μM ATP (2xKm) is added with 1 μCi [γ-33P]ATP and the reaction is incubated at room temperature for 45 minutes. Reactions are terminated with 3% phosphoric acid and transferred to P81 filter plates. Samples are washed in 1% phosphoric acid and 33P radioactivity is measured on a TopCount scintillation reader. In-house kinase assays are all carried out at 2xKm ATP concentrations. MPI-0479605 (500 nM) is also screened against a larger kinase panel. |
細胞研究 | Cells are treated for 3 or 7 days with various concentrations of MPI-0479605 and the GI50 is determined. Cell viability is measured with CellTiter-Glo. (Only for Reference) |
分子量 | 407.51 |
分子式 | C22H29N7O |
CAS No. | 1246529-32-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 58 mg/mL (142.3 mM)
Ethanol: 2 mg/mL (4.9 mM), Heating is recommended.
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
MPI-0479605 1246529-32-7 Apoptosis Cytoskeletal Signaling Kinesin Monopolar spindle 1 Inhibitor MPI 0479605 MPI0479605 inhibit Mps1 inhibitor