Powder: -20°C for 3 years | In solvent: -80°C for 1 year
MPTP hydrochloride is a precursor of MPP+, a dopamine neurotoxin with blood-brain barrier permeability. MPTP hydrochloride is toxic to dopaminergic neurons, which can lead to Parkinson's disease, and is widely used in the construction of animal models of Parkinson's disease.
説明 | MPTP hydrochloride is a precursor of MPP+, a dopamine neurotoxin with blood-brain barrier permeability. MPTP hydrochloride is toxic to dopaminergic neurons, which can lead to Parkinson's disease, and is widely used in the construction of animal models of Parkinson's disease. |
In vitro |
METHODS: Human neuroblastoma cells M17 were treated with MPTP hydrochloride (1-50 μM) for 48 h. The expression levels of target proteins were detected by Western Blot. RESULTS: MPTP hydrochloride promoted Tau protein phosphorylation in M17 cells. [1] METHODS: Neuroblastoma cell N2AB-1 and glioma cell C6 were treated with MPTP hydrochloride (0.33-33.7 μM) for 24 h, and the growth curves were examined. RESULTS: MPTP hydrochloride did not affect the cell number of actively growing N2AB-1 or C6 cells. [2] |
In vivo |
METHODS: To construct a subacute Parkinson's model, MPTP hydrochloride (30 mg/kg in 0.9% saline) was administered intraperitoneally to C57BL/6 mice once daily for five days. RESULTS: Subacute MPTP hydrochloride treatment did not cause significant motor deficits, although the dopaminergic system was severely impaired. mPTP hydrochloride significantly increased the level of α-synuclein and the number of astrocytes in the striatum and disrupted the blood-brain barrier in the substantia nigra pars compacta. [3] METHODS: To study the effects of different models on the behavior and pathology of a mouse model of Parkinson's disease, MPTP hydrochloride was used intraperitoneally to construct a subacute model group and a chronic model group. In the subacute model group, MPTP hydrochloride (30 mg/kg) was administered once daily for ten days. In the chronic model group, MPTP hydrochloride (30 mg/kg) was injected once every 3.5 days for five weeks for ten times. RESULTS: In the MPTP hydrochloride-induced subacute Parkinson's disease mouse model, there was a small loss of dopaminergic neurons in the midbrain, but there was no effect on the behavior.The MPTP hydrochloride-induced chronic Parkinson's disease mouse model lost a large number of dopaminergic neurons, which was accompanied by anxiety-like behaviors in addition to motor dysfunction. [4] |
別名 | MPTP-hydrochloride |
分子量 | 209.72 |
分子式 | C12H16ClN |
CAS No. | 23007-85-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 10mg/mL
DMSO: 5 mg/mL (23.84 mM)
You can also refer to dose conversion for different animals. 詳細
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