keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Methylene Blue (Tetramethylthionine chloride) (IC50 of 1.9 μM) inhibits tau filament formation. And it inhibits soluble guanylyl cyclase. It is also used as a dye in chromoendoscopy.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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50 mg | 在庫あり | ¥ 7,000 | |||
100 mg | 在庫あり | ¥ 10,000 | |||
200 mg | 在庫あり | ¥ 12,500 | |||
500 mg | 在庫あり | ¥ 17,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 11,500 |
説明 | Methylene Blue (Tetramethylthionine chloride) (IC50 of 1.9 μM) inhibits tau filament formation. And it inhibits soluble guanylyl cyclase. It is also used as a dye in chromoendoscopy. |
ターゲット&IC50 | Tau aggregation:1.9 μM, GC: |
In vitro | MB could inactivate nitric oxide directly by generating superoxide anions, it may inhibit the action of nitric oxide synthase itself. In addition to interfering with the nitric oxide–cyclic guanosine monophosphate pathway, there are MB directly activating calcium-dependent potassium channels and enhancing the release of noradrenaline from intracellular stores. |
In vivo | Methylene Blue decreases C-GMP and vascular smooth muscle relaxation by inhibiting guanylate cyclase. Pharmacokinetic studies in rodents showed that MB injects by intravascular resulting in a rapid and extensive accumulation of this drug in the central nervous system (CNS). When MB is injected intravenously, it selectively gathers in parathyroid glands, therefore is easy for the identification of structures during surgery. |
キナーゼ試験 | Isolated Integrin Binding Assays.:Purified integrin (1 μg/mL; 4℃) is used to coat 96-well microtitre plates, which are then blocked with bovine serum albumin (BSA) (3% in 1 mM CaCl2, 1 mM MgCl2,10 pM MnCl2, 100 mM NaC1,50 mM Tris-hydroxymethyl-aminomethane; pH 7.4), and incubated (3 h at 30 ℃) with biotinylated ligands (1 pg/mL in binding buffer: 0.1% BSA, 1 mM CaCl2,1 mM MgCl2, 10 μM MnCl2, 100 mM NaCl, 50 mM Tris-hydroxymethyl-aminomethane; pH 7.4) in the presence or absence of serially diluted peptides. After washing (3×5 min with binding buffer), the bound biotinylated ligand is detected with alkaline-phosphatase conjugated goat anti-biotin antibodies (1 μg/mL; 1 h, 37℃), using p-nitrophenyl phosphate as chromogen. Cyclo (-RGDfK) binding in the absence of competitor is defined as 100% signal; binding to blocked wells in the absence of integrin is defined as 0%. Concentrations of Cyclo (-RGDfK) required for 50% inhibition of signal (IC50 values) are estimated graphically. |
細胞研究 | Immature neuroblasts, isolated from the newborn rat subventricular zone, differentiate into γ-aminobutyric acid–mediated neurons are exposed at the sixth day in vitro to MB (10 μM) for 2h. Assess the survival and dendritic arbor architecture of these cells 48h after this treatment paradigm. |
動物実験 | MB (5 or 50 mg/kg) diluted in normal saline were injected intraarterially into Sprague- Dawley male rats over the 30s. |
別名 | methylthioninium chloride, CI-52015, Tetramethylthionine chloride, Basic Blue 9 |
分子量 | 319.85 |
分子式 | C16H18ClN3S |
CAS No. | 61-73-4 |
keep away from direct sunlight
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 3.2 mg/mL (10 mM)
DMSO: 1mg/ml, Sonication is recommended.
You can also refer to dose conversion for different animals. 詳細
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Methylene Blue 61-73-4 Cytoskeletal Signaling GPCR/G Protein Immunology/Inflammation Microbiology/Virology Neuroscience Guanylate cyclase Parasite NO Synthase Microtubule Associated Monoamine Oxidase antimalarial anxiolytic inhibit Methylthioninium methemoglobinemias cGMP CI 52015 Nitric oxide synthases methylthioninium chloride NOS Tetramethylthionine Chloride Inhibitor antinociception CI-52015 CI52015 Tetramethylthionine chloride antidepressant dye methylthioninium Chloride MAO vasopressor Basic Blue-9 Guanylate Cyclase Basic Blue9 Basic Blue 9 inhibitor