Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Molibresib (GSK525762) is an inhibitor of BET proteins (IC50: about 35 nM).
説明 | Molibresib (GSK525762) is an inhibitor of BET proteins (IC50: about 35 nM). |
ターゲット&IC50 | BET proteins:35 nM |
キナーゼ試験 | Fluorescence resonance energy transfer (FRET) titrations: Fluorescence resonance energy transfer (FRET) titrations. I-BET is titrated against BRD2 (200 nM), BRD3 (100 nM) and BRD4 (50 nM) in 50 mM HEPES pH7.5, 50 mM NaCl, 0.5 mM CHAPS in the presence of tetra-acetylated Histone H4 peptide (200 nM). After equilibrating for 1 hour, the bromodomain protein : peptide interaction is detected using FRET following the addition of 2 nM Europium cryptate labelled streptavidin and 10 nM XL-665-labelled anti-6His antibody in assay buffer containing 0.05% (v/v) BSA and 400 mM KF. Plates are read using an Envision Plate reader (excitation 320 nm, emission 615 nm and 665 nm). |
細胞研究 | CD4+ T cells are isolated from lymph nodes and spleens of 10- to 12-wk old mice and activated with plate bound anti-CD3 and anti-CD28 antibodies in the presence of indicated cytokines. I-BET-762 compounds is included during the 60–72 h of initial activation. Over the course of 5 d of T-cell culture and expansion, the compounds is diluted 12-fold relative to the starting concentrations.(Only for Reference) |
別名 | GSK 525762A, GSK525762, I-BET 762 |
分子量 | 423.9 |
分子式 | C22H22ClN5O2 |
CAS No. | 1260907-17-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 39 mg/mL (92 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 78 mg/mL (184 mM)
You can also refer to dose conversion for different animals. 詳細
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Molibresib 1260907-17-2 Chromatin/Epigenetic Epigenetic Reader Domain GSK525762A Inhibitor GSK 525762A GSK525762 inhibit GSK-525762 I-BET-762 I-BET762 GSK 525762 I-BET 762 inhibitor