Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Pregnanediol (5Beta-Pregnane-3Alpha,20alpha-Diol) is an inactive metabolite of PROGESTERONE by reduction at C5, C3, and C20 position. Pregnanediol(5Beta-Pregnane-3Alpha,20alpha-Diol) has two hydroxyl groups, at 3-alpha and 20-alpha. It is detectable in URINE after OVULATION and is found in great quantities in the pregnancy urine.
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10 mg | 在庫あり | ¥ 12,500 | |||
25 mg | 在庫あり | ¥ 23,000 | |||
50 mg | 在庫あり | ¥ 37,500 | |||
100 mg | 在庫あり | ¥ 62,500 | |||
200 mg | お問い合わせ | ¥ 81,000 |
説明 | Pregnanediol (5Beta-Pregnane-3Alpha,20alpha-Diol) is an inactive metabolite of PROGESTERONE by reduction at C5, C3, and C20 position. Pregnanediol(5Beta-Pregnane-3Alpha,20alpha-Diol) has two hydroxyl groups, at 3-alpha and 20-alpha. It is detectable in URINE after OVULATION and is found in great quantities in the pregnancy urine. |
キナーゼ試験 | Gelatin (0.1% (w/v) is added to standard LaemmLi acrylamide polymerization mixture. Tissue extract is mixed 1:2 with sample buffer [250 mM Tris-Cl pH 6.8, 10% (w/v) SDS, 20% (v/v) glycerol, 0.005% (w/v) bromphenol blue]. Serum is diluted 1:10 with electrophoresis buffer (2.5 mM Tris, 20 mM glycine, 0.005% SDS) and mixed 1:2 with sample buffer. Twenty μLs are loaded after 10-min incubation at room temperature without boiling. After electrophoresis at 90 V, the gels are soaked in 2.5% (w/v) Triton X-100, incubated 2 to 3 days at 37°C in gelatin digestion buffer [50 mM Tris-Cl, pH 8.0, 8 mM CaCl2, 10 mM ZnSO2, 0.02% (w/v) NaN3], stained in 0.05% Coomassie blue R-250 in acetic acid/methanol/water (1:4.5:4.5 by volume), destained in 10% acetic acid and 5% methanol, and scanned for lysis band intensity. The lysis band intensity is proportional to gelatinase activity and is quantified densitometrically by using One-Dimensional Scan software. The result, a number between 0.07 and 3.75, is normalized to the protein content by dividing the densitometry result with the relative optical density from the BCA protein assay kit result. The result is used for the analysis as the arbitrary unit. For the total MMP activity results of lysis bands of pro-MMP-9, active MMP-9, pro-MMP-2, and active MMP-2 are added. A protein size marker is used to determine the correct size. |
別名 | 5Beta-Pregnane-3Alpha,20alpha-Diol, Pregnandiol |
分子量 | 320.51 |
分子式 | C21H36O2 |
CAS No. | 80-92-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: Slightly soluble
You can also refer to dose conversion for different animals. 詳細
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Pregnanediol 80-92-2 Endocrinology/Hormones Metabolism Estrogen/progestogen Receptor Endogenous Metabolite measure NSC 1612 metabolic product 5Beta-Pregnane-3Alpha,20alpha-Diol Inhibitor progesterone 5b-Pregnane-3a,20a-Diol NSC1612 NSC-1612 urine NSC 47462 5β-Pregnane-3α,20α-Diol inhibit Pregnandiol NSC-47462 NSC47462 inhibitor