Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CCG-1423, a selective RhoA pathway inhibitor, suppresses SRF-mediated transcription.
説明 | CCG-1423, a selective RhoA pathway inhibitor, suppresses SRF-mediated transcription. |
ターゲット&IC50 | LRRK2 (WT):1.28 nM, LRRK2 (G2019S):1.85 nM |
In vivo | In H9c2 cells, CCG-1423 inhibits MRTF nuclear localization and completely blocks the activity of STARS proximal reporter genes. It also suppresses TGF-β-induced fibrogenesis in human colon myofibroblasts. Under the influence of CCG-1423 and LY294002, mouse embryonic stem cells differentiate into the mesendoderm. In melanoma cells (A375M2 and SK-Mel-147) overexpressing RhoC, CCG-1423 selectively inhibits cell proliferation. |
細胞研究 | Cells in normal culture medium are plated (2,000 per well) in a 96-well plate coated with laminin. After attachment, the medium is replaced with serum-free medium (0% FBS) with 30 μmol/L LPA with or without 300 nM CCG-1423. Fresh LPA with or without CCG-1423 is added at day 5 to ensure that LPA and compound are present throughout the experiment. On day 8, WST-1 reagent is added to the wells for 1 h and absorbance at 450 nm is read using a Victor plate reader.(Only for Reference) |
別名 | CCG1423 |
分子量 | 454.75 |
分子式 | C18H13ClF6N2O3 |
CAS No. | 285986-88-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 4 mg/mL (8.79 mM), Heating is recommended.
H2O: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 83 mg/mL (182.5 mM)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
CCG-1423 285986-88-1 Autophagy Cell Cycle/Checkpoint GPCR/G Protein MAPK LRRK2 Rho Ras PC-3 conformational restriction mice Inhibitor bioisostere CCG 1423 glucose tolerance metastasis Apoptosis CCG1423 inhibit prostate,serum inhibitor