Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CK-636 (CK-0944636), an Arp2/3 complex inhibitor, can inhibit actin polymerization induced by the human (IC50: 4 μM), fission yeast (IC50: 24 μM) and bovine (IC50: 32 μM) Arp2/3 complex, respectively.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
サンプルについてお問い合わせ | |||||
5 mg | 在庫あり | ¥ 7,000 | |||
10 mg | 在庫あり | ¥ 11,000 | |||
25 mg | 在庫あり | ¥ 19,000 | |||
50 mg | 在庫あり | ¥ 32,000 | |||
100 mg | 在庫あり | ¥ 45,500 | |||
500 mg | 在庫あり | ¥ 118,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 7,500 |
説明 | CK-636 (CK-0944636), an Arp2/3 complex inhibitor, can inhibit actin polymerization induced by the human (IC50: 4 μM), fission yeast (IC50: 24 μM) and bovine (IC50: 32 μM) Arp2/3 complex, respectively. |
ターゲット&IC50 | Arp2/3 complex (bovine):32 μM, Arp2/3 complex (fission yeast):24 μM, Arp2/3 complex (human):4 μM |
In vivo | CK-636 slows down cell migration by inhibiting the formation of lamellipodia at the leading edge of migrating T-cells. In live cells, it suppresses actin polymerization mediated by the Arp2/3 complex. In infected SKOV3 cells, CK-636 reduces the formation of actin filaments. Moreover, a majority of T-cells treated with CK-636 exhibit a turning event upon encountering a zigzag-shaped interface during a letter-shaped test experiment. |
キナーゼ試験 | HDAC inhibition assays: HDAC inhibition assays are performed by Reaction Biology Corp. using isolated human, recombinant full2length HDAC1 and -6 from a baculovirus expression system in Sf9 cells. An acetylated fluorogenic peptide,RHKKAc, derived from residues 379-382 of p53 is used as substrate. The reaction buffer is made up of 50 mM Tris-HCl pH 8.0, 127 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/mL BSA, and a final concentration of 1% DMSO. Compounds are delivered in DMSO and delivered to enzyme mixture with preincubation of 5-10 min followed by substrate addition and incubation for 2 h at 30°C. Trichostatin A and developer are added to quench the reaction and generate fluorescence, respectively. Dose-response curves are generated starting at 30 μM compound with three-fold serial dilutions to generate a 10-dose plot. IC50 values are then generated from the resulting plots, and the values expressed are the average of duplicate trials ± standard deviation. |
別名 | CK-0944636, CK 636 |
分子量 | 284.38 |
分子式 | C16H16N2OS |
CAS No. | 442632-72-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: 47 mg/mL (165.3 mM)
DMSO: 53 mg/mL (186.4 mM)
You can also refer to dose conversion for different animals. 詳細
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
CK-636 442632-72-6 Cytoskeletal Signaling Microtubule Associated CK 0944636 Inhibitor Actin-related protein 2/3 complex CK636 CK-0944636 CK0944636 Arp2/3 Complex inhibit CK 636 inhibitor