keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Cyclo(-RGDfK) (Cyclo (-RGDfK)) is an effective and specific αvβ3 integrin inhibitor.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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2 mg | 在庫あり | ¥ 13,000 | |||
5 mg | 在庫あり | ¥ 23,000 | |||
10 mg | 在庫あり | ¥ 41,500 | |||
25 mg | 在庫あり | ¥ 78,500 | |||
50 mg | 在庫あり | ¥ 126,000 | |||
100 mg | 在庫あり | ¥ 147,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 35,500 |
説明 | Cyclo(-RGDfK) (Cyclo (-RGDfK)) is an effective and specific αvβ3 integrin inhibitor. |
ターゲット&IC50 | αvβ3:0.94 nM. |
In vitro | Cyclo (-RGDfK-) has a high affinity for purified integrin (Kd: 41.70 nM). It is reacting with HEK293(β3) cells moderately. The micelles modified by Cyclo(-RGDfK-) show strong affinity to T-24 cells and strong inhibitory effect on the proliferation of T-24 cells. |
In vivo | In athymic mice bearing α(v)β(3)-integrin-positive C6 gliomas, Cyclo (-RGDfK-) modification induces less tumor metabolic activity, less tumor progression, fewer intratumoral vessels. |
キナーゼ試験 | Isolated Integrin Binding Assays.:Purified integrin (1 μg/mL; 4℃) is used to coat 96-well microtitre plates, which are then blocked with bovine serum albumin (BSA) (3% in 1 mM CaCl2, 1 mM MgCl2,10 pM MnCl2, 100 mM NaC1,50 mM Tris-hydroxymethyl-aminomethane; pH 7.4), and incubated (3 h at 30 ℃) with biotinylated ligands (1 pg/mL in binding buffer: 0.1% BSA, 1 mM CaCl2,1 mM MgCl2, 10 μM MnCl2, 100 mM NaCl, 50 mM Tris-hydroxymethyl-aminomethane; pH 7.4) in the presence or absence of serially diluted peptides. After washing (3×5 min with binding buffer), the bound biotinylated ligand is detected with alkaline-phosphatase conjugated goat anti-biotin antibodies (1 μg/mL; 1 h, 37℃), using p-nitrophenyl phosphate as chromogen. Cyclo (-RGDfK) binding in the absence of competitor is defined as 100% signal; binding to blocked wells in the absence of integrin is defined as 0%. Concentrations of Cyclo (-RGDfK) required for 50% inhibition of signal (IC50 values) are estimated graphically. |
別名 | Cyclo (-RGDfK) |
分子量 | 603.67 |
分子式 | C27H41N9O7 |
CAS No. | 161552-03-0 |
keep away from moisture
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 80 mg/mL(111.5 mM)
Ethanol: 84 mg/mL (117.04 mM)
DMSO: 100 mg/mL (139.33 mM)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Cyclo(-RGDfK) 161552-03-0 Cytoskeletal Signaling Integrin inhibit Microvasculature Cyclo (-RGDfK) Cyclo cancer tumoral cell αvβ3 integrin Cyclo(RGDfK) ligand Inhibitor -RGDfK Cyclo( RGDfK) inhibitor