Powder: -20°C for 3 years | In solvent: -80°C for 1 year
GSK2334470 is a novel PDK1 inhibitor (IC50: ~10 nM, in a cell-free assay), with no inhibitory at other close related AGC-kinases.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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サンプルについてお問い合わせ | |||||
5 mg | 在庫あり | ¥ 11,000 | |||
10 mg | 在庫あり | ¥ 18,500 | |||
25 mg | 在庫あり | ¥ 34,000 | |||
50 mg | 在庫あり | ¥ 62,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 20,000 |
説明 | GSK2334470 is a novel PDK1 inhibitor (IC50: ~10 nM, in a cell-free assay), with no inhibitory at other close related AGC-kinases. |
ターゲット&IC50 | PDK1:10 nM |
In vivo | The efficacy of the PDK1 inhibitor (PDKi) GSK2334470 was assessed in newborn BrafV600E::Pten?/? mice through systemic administration of 4-HT. Administering PDK1 twice weekly significantly inhibited pigmented lesions and concurrent melanomagenesis, alongside a notable reduction (~80%) in lung metastases and lymph node metastases, as quantified by H&E staining and S100 immunostaining, respectively. This outcome mirrors the effects observed with the genetic deletion of Pdk1[4]. |
キナーゼ試験 | FGFR1-4 Biochemical Assays: FGFR kinase inhibition assays are performed at KM for ATP. Picomolar to low nanomolar concentrations of FGFR proteins are incubated in 1× Kinase Reaction Buffer (KRB) with 1 μM of CSKtide and 50 to 250 of μM ATP at 25°C for 90 minutes in the presence or absence of a dosed concentration series of inhibitor. All reactions are terminated by the addition of Stop buffer, and plates are read on a Caliper EZReader2. IC50 values are fit with a four-parameter log[Inhibitor] versus response model with floating Hill Slope. |
細胞研究 | GSK2334470 is dissolved in DMSO and diluted with appropriate medium before use. To study the inhibitory effect of GSK2334470 on mTOR-S6K pathway, non-resistant cells and the resistant sublines are treated with GSK2334470 at 5 μM for 1.5 and 12 h in 10 % FBS medium with/without MK-2206 (5 μM)[2]. |
分子量 | 462.59 |
分子式 | C25H34N8O |
CAS No. | 1227911-45-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Ethanol: 83 mg/mL (179.4 mM)
DMSO: 83 mg/mL (179.4 mM)
You can also refer to dose conversion for different animals. 詳細
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GSK2334470 1227911-45-6 PI3K/Akt/mTOR signaling PDK GSK-2334470 Inhibitor GSK 2334470 PDK-1 inhibit inhibitor