Powder: -20°C for 3 years | In solvent: -80°C for 1 year
GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM).
説明 | GW 4064 is an effective farnesoid X receptor (FXR) agonist (EC50 =65 nM). |
ターゲット&IC50 | FXR:65 nM(EC50) |
In vitro | In Fisher rats, oral administration of GW 4064 (ED50 = 20 mg/kg) effectively reduces serum triglycerides. |
In vivo | In CV-1 cells transfected with mouse FXR expression vectors (EC50=80 nM) and in CV-1 cells transfected with human FXR expression vectors (EC50=90 nM), GW 4064 functions as a selective non-steroidal FXR agonist, effectively exerting agonistic effects. |
細胞研究 | GW 4064 is dissolved in DMSO and stored, and then diluted with appropriate media before use[2]. Mouse liver cells (BNL CL.2) are maintained in a humidified incubator under 5% CO2 at 37°C in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin. When cells are divided into six-well plates and reach ~90% confluence, sub-confluent cells are washed three times with phosphate buffered saline (PBS) and replaced with serum-free DMEM supplemented with 1% fatty acid-free BSA. Oleic acid (final concentration 500 μM) and GW4064 at various concentrations are added and incubated for 24 h. Cells are then fixed with 4% formaldehyde for Oil Red O staining or harvested for protein and western blot analysis[2]. |
分子量 | 542.84 |
分子式 | C28H22Cl3NO4 |
CAS No. | 278779-30-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 50 mg/mL (92.11 mM)
Ethanol: 5.4 mg/mL (10 mM)
You can also refer to dose conversion for different animals. 詳細
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GW 4064 278779-30-9 Autophagy Metabolism FXR Inhibitor inhibit NR1H4 GW-4064 GW4064 inhibitor