Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Itanapraced (CHF5074), a γ-secretase modulator, reduces Aβ42/40 secretion (IC50: 3.6/18.4 μM).
説明 | Itanapraced (CHF5074), a γ-secretase modulator, reduces Aβ42/40 secretion (IC50: 3.6/18.4 μM). |
ターゲット&IC50 | Aβ40:18.4 μM, Aβ42:3.6μM |
キナーゼ試験 | GSK-3β activity assay: IC50 of new synthesised compound IM-12 to GSK-3β is determined by a luminometric GSK-3β activity assay. Briefly, compounds are tested in different concentrations diluted in assay buffer containing final concentrations of: 4 mM MOPS pH 7.2; 0.4 mM EDTA; 1 mM EGTA; 2.5 mM β-glycerophosphate; 4 mM MgCl2; 40 μM BSA; 0.05 mM DTT. Four microlitres of diluted compounds are added to 25 μM pGS-2 peptide substrate, 20 ng recombinant GSK-3β and 1 μM ATP to a total assay volume of 40 μl. The enzymatic reaction is stopped after 30 min of incubation at 30 °C by adding 40 μl KinaseGlo. The luminometric signal is allowed to stabilise for 10 min and the measured with a Glomax? 96 Microplate Luminometer. |
別名 | CHF5074, CSP-1103 |
分子量 | 325.16 |
分子式 | C16H11Cl2FO2 |
CAS No. | 749269-83-8 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 37 mg/mL
You can also refer to dose conversion for different animals. 詳細
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Itanapraced 749269-83-8 Neuroscience Proteases/Proteasome Stem Cells Gamma-secretase Beta Amyloid CSP 1103 Inhibitor CHF 5074 Gamma secretase γ-secretase CSP1103 CHF5074 CSP-1103 inhibit CHF-5074 inhibitor