Powder: -20°C for 3 years | In solvent: -80°C for 1 year
RITA (NSC-652287)(NSC-652287) induced cross-links of both DNA-DNA and DNA-protein with no detectable DNA single-strand breaks. RITA, like nutlin-3, can disrupt the p53/Mdm2 interaction.
説明 | RITA (NSC-652287)(NSC-652287) induced cross-links of both DNA-DNA and DNA-protein with no detectable DNA single-strand breaks. RITA, like nutlin-3, can disrupt the p53/Mdm2 interaction. |
ターゲット&IC50 | p53dN:1.5 nM (Kd) |
キナーゼ試験 | The inhibition profile of cabozantinib against a broad panel of 270 human kinases is determined using luciferase-coupled chemiluminescence,?33P-phosphoryl transfer, or AlphaScreen technology. Recombinant human full-length, glutathione?S-transferase tag, or histidine tag fusion proteins are used, and half maximal inhibitory concentration (IC50) values are determined by measuring phosphorylation of peptide substrate poly (Glu, Tyr) at ATP concentrations at or below the?Km?for each respective kinase. The mechanism of kinase inhibition is evaluated using the AlphaScreen Assay by determining the IC50?values over a range of ATP concentrations. |
細胞研究 | Examination to assess susceptibility of cells to RITA (0.1 nM - 1 mM) is done using the XTT assay. Cells are inoculated into 96-well flat-bottom plates at a density of 1500 cells per well and incubated for 24 hours at 37 °C in a humidified 5% CO2 5% air atmosphere. Serial concentrations of RITA in DMSO are added to the wells, and sensitivity is determined 48 hours after the addition of RIT(Only for Reference) |
別名 | NSC 652287, RITA (NSC 652287) |
分子量 | 292.37 |
分子式 | C14H12O3S2 |
CAS No. | 213261-59-7 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 29.2 mg/mL (100 mM)
Ethanol: 7.3 mg/mL (25 mM)
You can also refer to dose conversion for different animals. 詳細
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RITA 213261-59-7 Apoptosis Autophagy DNA Damage/DNA Repair Mdm2 DNA Alkylator/Crosslinker p53 MDM-2/p53 inhibit NSC-652287 NSC 652287 RITA (NSC 652287) NSC652287 Inhibitor inhibitor