Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SP2509 is a specific histone demethylase LSD1 inhibitor(IC50 =13 nM).
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
サンプルについてお問い合わせ | |||||
5 mg | 在庫あり | ¥ 12,000 | |||
10 mg | 在庫あり | ¥ 18,500 | |||
25 mg | 在庫あり | ¥ 33,000 | |||
50 mg | 在庫あり | ¥ 56,000 | |||
100 mg | 在庫あり | ¥ 91,500 | |||
500 mg | 在庫あり | ¥ 215,000 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 12,500 |
説明 | SP2509 is a specific histone demethylase LSD1 inhibitor(IC50 =13 nM). |
ターゲット&IC50 | LSD1:13 nM |
キナーゼ試験 | SP2509 activity assays: Test compounds are diluted to 20 × the desired test concentration in 100% DMSO and 2.5?μL of the diluted drug sampleis added to a black 384-well plate. The LSD1 enzyme stock is diluted 17-fold with assay buffer and 40?μL of the diluted LSD1 enzyme is added to the appropriate wells. Substrate, consisting of horseradish peroxidase, dimethyl K4 peptide corresponding to the first 21 amino acids of the N-terminal tail of histone H3, and 10-acetyl-3,7-dihydroxyphenoxazine is then added to wells. Resorufin is analyzed on an Envision plate reader with an excitation wavelength of 530?nm and an emission wavelength of 595?nm. The activity of SP2509 on the other oxidases is determined by using commercially available kits. The glucose oxidase activity (which also noncovalently binds FAD in an elongate conformation), is determined using the glucose oxidase kit. The MAO assays are performed using the MAO-glo kit with MAO-A and MAO-B. |
細胞研究 | Cultured AML cells are treated with SP2509 and/or PS for 96?h. At the end of treatment, cells are washed free of the drugs and 500 cells per condition are plated in methylcellulose and incubated at 37?°C. Colony formation is measured 7–10 days after plating.(Only for Reference) |
別名 | HCI-2509 |
分子量 | 437.9 |
分子式 | C19H20ClN3O5S |
CAS No. | 1423715-09-6 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 36 mg/mL (82.2 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
H2O: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
SP2509 1423715-09-6 Apoptosis Chromatin/Epigenetic Histone Demethylase HCI 2509 SP 2509 SP-2509 inhibit Inhibitor HCI-2509 HCI2509 inhibitor