Powder: -20°C for 3 years | In solvent: -80°C for 1 year
USP7/USP47 inhibitor (USP7/47 inhibitor-1) is a selective ubiquitin-specific protease 7/47 (USP7/USP47) inhibitor, with EC50s of 0.42 μM and 1.0 μM, respectively.
説明 | USP7/USP47 inhibitor (USP7/47 inhibitor-1) is a selective ubiquitin-specific protease 7/47 (USP7/USP47) inhibitor, with EC50s of 0.42 μM and 1.0 μM, respectively. |
ターゲット&IC50 | USP47:1.0 μM(EC50), USP7:0.42 Μm(EC50) |
In vitro | USP7/USP47 inhibitor does not inhibit caspase 3, calpain 1, 20S proteasome, and a panel of representative USPs (USP2, USP5, USP8, USP21, and USP28; EC50 > 31.6 μM). USP7/USP47 inhibitor inhibits the growth of HCT-116 cells with an EC50 of 7.6 μM. |
キナーゼ試験 | The cloning, expression and purification of USP21 from BL21 (DE3) bacteria are performed using standard molecular biology techniques. USP2, USP5, USP7, USP8, USP28, USP47, Ub-PLA2 (Ub-CHOP) and Ub-EKL (Ub-CHOP2) are generated. Caspase 3 and the caspase 3 substrate DEVD-Rh110 are used. Deubiquitylating enzyme, cathepsin B and 20S proteasome chymotrypsin like protease activities are measured. Caspase 3 activity is determined using a similar protocol. Briefly, dose ranges of compound (including USP7/USP47 inhibitor) are incubated with caspase 3 for 30 minutes before the addition of DEVD-Rh110 and reading on a fluorometric plate reader using excitation and emission maxima of 485 nm and 531 nm respectively. The final concentrations of caspase 3 and DEVD-Rh110 are 2 nM and 100 nM respectively |
別名 | USP7/47 inhibitor-1 |
分子量 | 484.4 |
分子式 | C18H11Cl2N3O3S3 |
CAS No. | 1247825-37-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 45 mg/mL (92.9 mM)
You can also refer to dose conversion for different animals. 詳細
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USP7/USP47 inhibitor 1247825-37-1 Cell Cycle/Checkpoint DNA Damage/DNA Repair Ubiquitination DUB USP-7/USP47 inhibitor Deubiquitinase inhibit Inhibitor USP7/47 inhibitor-1 DUBs inhibitor