Powder: -20°C for 3 years | In solvent: -80°C for 1 year
OTSSP167 (OTS167) is an orally available inhibitor of maternal embryonic leucine zipper kinase (MELK) with potential antineoplastic activity.
説明 | OTSSP167 (OTS167) is an orally available inhibitor of maternal embryonic leucine zipper kinase (MELK) with potential antineoplastic activity. |
ターゲット&IC50 | MELK:0.41 nM |
キナーゼ試験 | in vitro kinase assay: MELK recombinant protein (0.4 μg) is mixed with 5 μg of each substrate in 20 μL of kinase buffer containing 30 mM Tris-HCl (pH), 10 mM DTT, 40 mM NaF, 10 mM MgCl2, 0.1 mM EGTA with 50 μM cold-ATP and 10 Ci of [γ-32P]ATP for 30 min at 30 °C. The reaction is terminated by addition of SDS sample buffer and boiled for 5 min prior to SDS-PAGE. The gel is dried and autoradiographed with intensifying screens at room temperature. OTSSP167 (final concentration of 10 nM) is dissolved in DMSO and added to kinase buffer before the incubation. |
細胞研究 | Cell Counting Kit-8(Only for Reference) |
別名 | MELK inhibitor, OTS167 |
分子量 | 487.42 |
分子式 | C25H28Cl2N4O2 |
CAS No. | 1431697-89-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 7.22 mg/ml (14.82 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. 詳細
bottom
Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
OTSSP167 1431697-89-0 PI3K/Akt/mTOR signaling MELK MELK inhibitor inhibit Maternal embryonic leucine zipper kinase Inhibitor OTS-167 OTSSP 167 OTS 167 OTS167 OTSSP-167 inhibitor