Powder: -20°C for 3 years | In solvent: -80°C for 1 year
(-)-Parthenolide ((-)-Parthenolide), an inhibitor of the Nuclear Factor-κB Pathway, also promotes the ubiquitination of MDM2 and activates p53 cellular functions.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
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50 mg | 在庫あり | ¥ 11,500 | |||
100 mg | 在庫あり | ¥ 16,000 | |||
200 mg | 在庫あり | ¥ 27,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 11,500 |
説明 | (-)-Parthenolide ((-)-Parthenolide), an inhibitor of the Nuclear Factor-κB Pathway, also promotes the ubiquitination of MDM2 and activates p53 cellular functions. |
In vitro | Parthenolide (PTL) has a dose-dependent growth inhibition effect on NSCLC cells Calu-1, H1792, A549, H1299, H157, and H460. Parthenolide can induce cleavage of apoptotic proteins such as CASP8, CASP9, CASP3 and PARP1 both in concentration- and time-dependent manner in tested lung cancer cells which indicates that apoptosis is trigged after Parthenolide exposure. Besides induction of apoptosis, Parthenolide also induces G0/G1 cell cycle arrest in a concentration-dependent manner in A549 cells and G2/M cell cycle arrest in H1792 cells[2]. |
In vivo | Parthenolide, an HDAC inhibitor with anti-inflammatory properties, demonstrated significant anti-apoptotic effects in Phb1 KO hepatocytes. This compound, when administered alongside TSA, increased FXR levels while decreasing CYP7A1, HDAC4, TNFα, TRAIL, and Bax levels. This suggests that Parthenolide's specific HDAC inhibition might reduce bile acid toxicity, thereby attenuating the apoptotic response in Phb1 KO hepatocytes. Remarkably, Parthenolide also offered protection against liver injury in Phb1 KO mice post-bile duct ligation (BDL), as evidenced by a reduction in mortality rates, lower apoptotic responses, decreased necrotic areas, reduced Tunel-staining, and significantly lower ALT (8431±957 vs. 4225±210 U/L) and AST (4805±300 vs. 2242±438 U/L) levels compared to control Phb1 KO mice. |
細胞研究 | Parthenolide (PTL) is dissolved in DMSO and diluted with appropriate media[2]. Cells are seeded in 96-well plates and treated on the second day with the given concentration of Parthenolide (0, 5, 10, 20 μM) for another 48 hours and then subjected to SRB or MTT assay. For SRB assay, live cell number is estimated as described earlier. After treatment, the medium is discarded firstly. In order to fix the adherent cells, 100 μL of cold trichloroacetic acid (10% (w/v)) are adding to each well and incubating at 4°C for at least 1 hour. The plates are then washed five times with deionized water and dried in the air. Each well are then added with 50 μL of SRB solution (0.4% w/v in 1% acetic acid) and incubated for 5 min at room temperature. The plates are washed five times with 1% acetic acid to remove unbound SRB and then air dried. The residual bound SRB is solubilized with 100 μL of 10 mM Tris base buffer (pH 10.5), and then read using a microtiter plate reader at 495 nm. The MTT assay is executed. 20 μL MTT (5 mg/mL) are added to each sample and incubate at 37°C for 4 h, then 100 μL solubilization solution are added. Cell viability is determined at 595 nm[2]. |
植物由来 |
別名 | (-)-Parthenolide |
分子量 | 248.32 |
分子式 | C15H20O3 |
CAS No. | 20554-84-1 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 55 mg/mL (221.49 mM)
Ethanol: 50 mg/mL (201.35 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
Parthenolide 20554-84-1 Apoptosis Autophagy Chromatin/Epigenetic DNA Damage/DNA Repair NF-Κb Mitophagy NF-κB HDAC inhibit (-)-Parthenolide Nuclear factor-kappaB Mitochondrial Autophagy Nuclear factor-κB Inhibitor inhibitor