Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Rigosertib sodium (ON-01910), a non-ATP-competitive inhibitor of PLK1(IC50=9 nM), exhibits 30-fold higher specificity activity over Plk2 and no effect on Plk3.
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2 mg | 在庫あり | ¥ 8,000 | |||
5 mg | 在庫あり | ¥ 13,000 | |||
10 mg | 在庫あり | ¥ 20,500 | |||
25 mg | 在庫あり | ¥ 34,000 | |||
50 mg | 在庫あり | ¥ 55,000 | |||
100 mg | 在庫あり | ¥ 91,000 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 13,500 |
説明 | Rigosertib sodium (ON-01910), a non-ATP-competitive inhibitor of PLK1(IC50=9 nM), exhibits 30-fold higher specificity activity over Plk2 and no effect on Plk3. |
ターゲット&IC50 | CDK1:260 nM (IC50), Fyn:182 nM (IC50), PDGFR:18 nM (IC50), FLK1:42 nM (IC50), PLK1:9 nM (IC50), PLK2:260 nM (IC50), BCR-ABL:32 nM (IC50), Src:155 nM (IC50) |
キナーゼ試験 | In vitro enzyme assays for PLK1: Recombinant PLK1 (10 ng) is incubated with different concentrations of Rigosertib in a 15 μL reaction mixture (50 mM HEPES, 10 mM MgCl2, 1 mM EDTA, 2 mM Dithiothreitol, 0.01% NP-40 [pH 7.5]) for 30 min at room temperature. Kinase reactions are performed for 20 min at 30 °C in a volume of 20 μL (15 μL enzyme + inhibitor, 2 μL 1 mM ATP), 2 μL of γ32P-ATP (40 μCi), and 1 μL of recombinant Cdc25C (100 ng) or casein (1 μg) substrates. Reactions are terminated by boiling for 2 min in 20 μL of 2× Laemmli buffer. Phosphorylated substrates are separated by 18% SDS-PAGE. The gels are dried and exposed to X-ray film for 3-10 min. |
細胞研究 | Cells are grown in either DMEM or RPMI supplemented with 10% fetal bovine serum and 1 unit/mL penicillin-streptomycin solution. Tumor cells are plated into six-well dishes at a density of 1 × 105cells/mL/well, and Rigosertib is added 24 hours later at various concentrations. Cell counts are determined from duplicate wells after 96-hour of treatment. The total number of viable cells is determined by trypan blue exclusio(Only for Reference) |
別名 | Rigosertib, Estybon, ON-01910 |
分子量 | 473.47 |
分子式 | C21H24NNaO8S |
CAS No. | 592542-60-4 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 87 mg/mL (183.7 mM)
Ethanol: < 1 mg/mL (insoluble or slightly soluble)
DMSO: 88 mg/mL (185.9 mM)
You can also refer to dose conversion for different animals. 詳細
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Rigosertib sodium 592542-60-4 Apoptosis Cell Cycle/Checkpoint PI3K/Akt/mTOR signaling PLK PI3K Rigosertib Estybon inhibit Phosphoinositide 3-kinase ON-01910 Inhibitor ON01910 ON 01910 Polo-like Kinase (PLK) inhibitor