Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Pamidronic acid Leads to Bone Necrosis via Suppression of Wnt/β-Catenin Signaling in Human Bone Marrow Mesenchymal Stem Cells
説明 | Pamidronic acid Leads to Bone Necrosis via Suppression of Wnt/β-Catenin Signaling in Human Bone Marrow Mesenchymal Stem Cells |
In vitro | Pamidronate disodium inhibited Wnt and β-catenin signaling, which controls osteogenic differentiation in BMMSCs. Wnt3a, a Wnt and β-catenin signaling activator, reversed the negative effects caused by pamidronate disodium to salvage the osteogenic defect in BMMSCs[1]. |
細胞研究 | Primary human BMMSCs were isolated from the mandible and marrow tissue. A proliferation assay was performed to determine the experimental concentration of pamidronate disodium. Alkaline phosphatase (ALP) activity, ALP staining, and Alizarin red S (ARS) staining were assessed after treatment with pamidronate disodium (0, 0.1, 0.5, 1, 5, 10 μg/mL). Quantitative real-time polymerase chain reaction and western blotting specific for Wnt and β-catenin signaling genes or proteins were performed after treatment with pamidronate disodium 0.5 μg/mL. Wnt3a was used to observe the osteogenic differentiation of BMMSCs during treatment with pamidronate disodium 0.5 μg/mL[1]. |
分子量 | 235.07 |
分子式 | C3H11NO7P2 |
CAS No. | 40391-99-9 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
H2O: 6 mg/mL (25.52 mM), Sonication and heating are recommended.
You can also refer to dose conversion for different animals. 詳細
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Pamidronic acid 40391-99-9 Cytoskeletal Signaling Stem Cells Wnt/beta-catenin inhibit Inhibitor β-catenin Beta catenin Wnt inhibitor