Powder: -20°C for 3 years | In solvent: -80°C for 1 year
SKL2001, an agonist of the Wnt/β-catenin pathway, can disrupt the Axin/β-catenin interaction.
説明 | SKL2001, an agonist of the Wnt/β-catenin pathway, can disrupt the Axin/β-catenin interaction. |
In vitro | SKL2001 upregulated β-catenin responsive transcription by increasing the intracellular β-catenin protein level and inhibited the phosphorylation of β-catenin at residues Ser33/37/Thr41 and Ser45, which would mark it for proteasomal degradation, without affecting CK1 and GSK-3β enzyme activities. SKL2001 disrupted the Axin/β-catenin interaction, which is a critical step for CK1- and GSK-3β-mediated phosphorylation of β-catenin at Ser33/37/Thr41 and Ser45. The treatment of mesenchymal stem cells with SKL2001 promoted osteoblastogenesis and suppressed adipocyte differentiation, both of which were accompanied by the activation of Wnt/β-catenin pathway. SKL2001 did not affect either NF-κB or p53 reporter activity and inhibits β-catenin phosphorylation without affecting GSK-3β activity[1]. |
細胞研究 | The HEK293 reporter and control cell lines were established. The HEK293 reporter cells were inoculated into 384-well plates at 10 000 cells per well and grown for 24 h. Next, each compound in the chemical library (~270 000) was added to at a final concentration of 20 μM. After 15 h, the plates were assayed for firefly luciferase activity.(Only for Reference) |
分子量 | 286.29 |
分子式 | C14H14N4O3 |
CAS No. | 909089-13-0 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 53 mg/mL (185.1 mM)
Ethanol: 53 mg/mL (185.1 mM)
H2O: < 1 mg/mL (insoluble or slightly soluble)
You can also refer to dose conversion for different animals. 詳細
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SKL2001 909089-13-0 Cytoskeletal Signaling Stem Cells Wnt/beta-catenin Inhibitor Wnt Beta catenin β-catenin SKL-2001 inhibit SKL 2001 inhibitor