Powder: -20°C for 3 years | In solvent: -80°C for 1 year
WHI-P154 (Jak3 inhibitor ii) is a potent JAK3 inhibitor.
パッケージサイズ | 在庫状況 | 単価(税別) | |||
---|---|---|---|---|---|
サンプルについてお問い合わせ | |||||
2 mg | 在庫あり | ¥ 8,000 | |||
5 mg | 在庫あり | ¥ 13,000 | |||
10 mg | 在庫あり | ¥ 20,500 | |||
25 mg | 在庫あり | ¥ 42,500 | |||
50 mg | 在庫あり | ¥ 80,000 | |||
100 mg | 在庫あり | ¥ 130,500 | |||
1 mL * 10 mM (in DMSO) | 在庫あり | ¥ 13,500 |
説明 | WHI-P154 (Jak3 inhibitor ii) is a potent JAK3 inhibitor. |
ターゲット&IC50 | EGFR:4 nM, VEGFR:100 nM, JAK3:1.8 μM, Src:100 nM |
キナーゼ試験 | Kinase assays: WHI-P154 is tested in kinase assays. The panel of kinases is selected to broadly cover the kinome, providing a good approximation of specificity. For all kinases, recombinant rat (IKKβ) or human (all others), full-length or GST-kinase domain fusion proteins, are used. WHI-P154 is inactive (concentration that inhibits response by 50% [IC50] > 30 μM) for the following kinases: AKT, AuroraA, cdk2, cdk6, CHK1, FGFR1, GSK3b, IKKb, IKKi, INSR, MAPK1, MAPKAP-K2, MASK, MET, PAK4, PDK1, PKCb, ROCK1, TaoK3, TrkA. |
細胞研究 | Cells are seeded into a 96-well plate at a density of 2.5×104 cells/well and incubated for 36 h at 37 ℃ before drug exposure. On the day of treatment, culture medium is carefully aspirated from the wells and replaced with fresh medium containing the quinazoline compounds WHI-P154 at concentrations ranging from 0.1 μM to 250 μM. Triplicate wells are used for each treatment. The cells are incubated with the compound for 24hours to 36hours at 37 ℃ in a humidified 5% CO2 atmosphere. To each well, 10 μL of MTT (final concentration, 0.5 mg/mL) is added, and the plate are incubated at 37 ℃ for 4 h. Than solubilized overnight at 37 ℃ in a solution containing 10% SDS in 0.01 M HCL. The absorbance of each well is measured in a microplate reader at 570 nm.(Only for Reference) |
別名 | Jak3 inhibitor ii |
分子量 | 376.2 |
分子式 | C16H14BrN3O3 |
CAS No. | 211555-04-3 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 37.6 mg/mL (100 mM)
You can also refer to dose conversion for different animals. 詳細
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc.
WHI-P154 211555-04-3 Angiogenesis Apoptosis Chromatin/Epigenetic JAK/STAT signaling Stem Cells Tyrosine Kinase/Adaptors JAK Src EGFR VEGFR Janus kinase Jak3 inhibitor ii WHI-P-154 WHI-P 154 ErbB-1 inhibit Epidermal growth factor receptor HER1 WHIP154 Inhibitor WHI P154 inhibitor